Journal: bioRxiv
Article Title: Immunomodulatory effect of mycobacterial outer membrane vesicles coated nanoparticles
doi: 10.1101/2022.06.07.495204
Figure Lengend Snippet: THP-1 cells treated with OMV-AuNP showed significantly increased cytokine release. The concentration of ( A ) hIL-8 and ( B ) hIL-6 cytokines released into the media by THP-1 cells as determined by ELISA. Mouse BMDM cells treated with OMV-AuNP showed pronounced release of cytokines ( C ) mIL-6 and ( D ) mTNF-α in the media as determined by ELISA. Data in the graphs represent the mean ± s.d. **** p < 0.0001 and ** p < 0.01 were determined by ordinary one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparison test.
Article Snippet: For quantifying the cytokines released from THP-1 cells, hIL-8 and hIL-6 were estimated using their respective ELISA kits (Peprotech) according to the manufacturer’s instructions.
Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Comparison